PCR petB

Janelle Pokorski and Tenesha Cook used PCR to amplify the petB region of DNAs extracted from four orchid species: Sedirea japonica, Podangis dactyloceras, Cleisostoma teres, and Papilionanthe vandarum. A total of nine samples (two for each species and a negative control) were then used for gel electrophoresis to obtain the following results:

Gel Electrophoresis of DNAs 3-2, 3-10, 300, & 364

Lane	ID	Species or Marker	Success
1	n/a	Molecular Mass Ruler	n/a
2	n/a	Negative Control	n/a
3	3-2	Sedirea japonica	++
4	3-2	Sedirea japonica	++
5	3-10	Podangis dactyloceras	++
6	3-10	Podangis dactyloceras	++
7	300	Cleisostoma teres	++*
8	300	Cleisostoma teres	++
9	364	Papilionanthe vandarum	++
10	364	Papilionanthe vandarum	++