harsh198819

In this research, an in vivo test system is assembled in order to perform a study on group II intron splicing. Group II intron splicing is studied because it tends to interrupt reading frames in plastid and bacteria. The gene, udiA, is a chimeric reporter gene which is transcribed in tobacco plants. This gene is formed by fusing the tobacco plastid DNA region (which contains the atpF gene intron) with the region of the plastid that codes for the udiA chimeric reporter gene. The atpF intron is specifically chosen for this study because it is the smallest of the introns found in tobacco plastid DNA. Furthermore, a northern blot analysis is performed to confirm that the correct intron is removed. Upon viewing the results, it was discovered that the atpF gene region contained adequate amounts of nucleotides for both upstream and downstream exons to correctly splice a group II intron. Through this research, it was concluded that the plasmid transformants were not affected by atpF splicing. This indicated that the atpF intron excision is not the reason for the ineffective splicing ability of group II intron by the chimeric reporter gene udiA in tobacco plastids.

Reference:

Bock, Ralph, and Pal Maliga. 1995. Correct Splicing of a Group II Intron from a Chimeric Reporter Gene Transcript in Tobacco Plastids. Nucleic Acids Research 23.13: 2544-2547.

http://www.mendeley.com/research/correct-splicing-group-ii-intron-chimeric-reporter-gene-transcript-tobacco-plastids/

Edited: 12/14/2011

Sedirea japonica L. Linden & Rchb. f. is a species which belongs to the genus Sediera, sub-tribe Aeridinea. Native to the Nago-mountain of southern japan, where it’s commonly know as “Nago-ran”. They grow as epiphytes in the wild forest of the mountains at elevations of about 330 ft. Ideal environment for the growth of these plants is they require moderate light with cool to intermediate warm temperature of 68 to 80 degrees. With a bearing fragrance of lemon-citrus in them, the flowers bloom during the months of January to March with a cream white and hint of purple in their petals. Normal heights for these plants are 15cm with growing space from 6-15cm and ideal pH of the media between 6.1 to 6.5.